Technology is constantly evolving, and so is the equipment used in small to large labs, research facilities, and hospitals. While Sanger sequencing used to be the cutting-edge technology, today the Illumina DNA sequencer line offers next-generation sequencing ability that is ideally suited to most applications.
The Advantages
The difference in the two technologies allows for shorter testing times, smaller sample sizes and higher accuracy of sequencing at faster rates. The Illumina DNA sequencer technology uses a process known as clonal amplification which is combined with chemistry through synthesis. With this combination, it is possible for the equipment to both identify the DNA bases while also forming the chain of nucleic acids.
The DNA bases in the nucleic acid chain are each identified with a unique fluorescent marker which emits a signal. Monitoring of these markers through the signals allows for rapid and accurate DNA sequencing.
Use in Labs
The next-generation sequencing technology used in the Illumina DNA sequencer is applicable to any type of organism’s DNA. It is essential for any type of biological research where DNA sequencing is required. The technology can be used on small sections or area of the genome or on the complete genome based on the specifics of the research requirements.
Through parallel sequencing, large sections of the DNA can be replicated with speed and accuracy not possible through Sanger-sequencing methods. This also offers a simple way to provide accurate measurements of the DNA through the intensity of the signals emitted, which can be accurately captured and recorded in real time.
With the capacity to work in high throughput labs or to be used in smaller testing facilities, there is an Illumina next-generation sequencer right for any facility. Small to large models are designed to be operator-friendly and easily integrated into any testing lab.
